线粒体热化学研究Ⅱ线粒体代谢活性的热化学研究

应用科学学报 ›› 1997, Vol. 15 ›› Issue (4): 469-473.

线粒体热化学研究Ⅱ线粒体代谢活性的热化学研究

刘义, 冯英, 谢昌礼, 周培疆, 屈松生, 朱英国, 周涵韬

武汉大学

收稿日期:1995-08-27 修回日期:1996-01-02 出版日期:1997-12-31 发布日期:1997-12-31
作者简介:刘义:博士,武汉大学化学系,武汉 430072
基金资助:
国家自然科学基金

THERMOCHEMICAL STUDY OF MITOCHONDRIA ⅡTHERMOCHEMICAL STUDY OF THE ACTIVITY OF MITOCHONDRIAL METABOLISM

LIU YI, FENG YING, XIE CHANGLI, ZHOU PEIJIANG, QU SONGSHENG, ZHU YINGGUO, ZHOU HANTAO

Wuhan University, Wuhan 430072

Received:1995-08-27 Revised:1996-01-02 Online:1997-12-31 Published:1997-12-31

摘要/Abstract

摘要： 用LKB2277生物活性检测系统,测定了水稻线粒体代谢的产热曲线,研究了水稻线粒体体外代谢的热力学和动力学,建立了线粒体代谢活性增长期的热动力学方程,得到了一些热力学和动力学参数.

关键词: 线粒体, 代谢, 微量热法, 热化学, 热动力学

Abstract: The metabolic thermogenesis curves of the metabolism of the mitochondria isolated from rice have been determined by using an LKB2277 Bioactivity Monitor. The thermodynamics and kinetics of the rice mitochondrial metabolism out of the cell have been studied. The metabolic thermogenesis curves of the rice mitochondria appeared four different phases. If the rice mitochondria have been laid up for different time, the metabolic thermogenesis curves and the kinetic behaviours of the rice mitochondria are different. Some thermodynamic values and kinetic paramters, as well as some new experimental thermokinetic equations in the activity increasing phase of the rice mitochondria,have been calculated and established.

Key words: microcalorimetry, thermochemistry, rice mitochondria, metabolism, thermokinetics

刘义, 冯英, 谢昌礼, 周培疆, 屈松生, 朱英国, 周涵韬. 线粒体热化学研究Ⅱ线粒体代谢活性的热化学研究[J]. 应用科学学报, 1997, 15(4): 469-473.

LIU YI, FENG YING, XIE CHANGLI, ZHOU PEIJIANG, QU SONGSHENG, ZHU YINGGUO, ZHOU HANTAO. THERMOCHEMICAL STUDY OF MITOCHONDRIA ⅡTHERMOCHEMICAL STUDY OF THE ACTIVITY OF MITOCHONDRIAL METABOLISM[J]. Journal of Applied Sciences, 1997, 15(4): 469-473.