中文

Journal of Applied Sciences ›› 1984, Vol. 2 ›› Issue (2): 178-183.

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THE PREPARATION OF HIGH ACTIVITY ALKALIN PHOSPHATASE FROM CALF INTESTINAL MUCOSE

JIANG YIZHONG1, QIOU YAOJIU1, YANG YANZHONG2, QUO CHUNXIANG3   

  1. 1. Shanghai University of Science and Technology;
    2. East China Institute of Chemical Technology;
    3. Shanghai Medical Laboratory
  • Received:1982-04-27 Online:1984-06-30 Published:1984-06-30

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Abstract: This paper presents a method for the preparation of alkaline phosphatase (EC 3.1.3.1) from calf intestinal mueose. The method involves extraction of the mucose with butanol, fractionation with acetone and then (NH4)2SO4, DEAE-cellulose ion-exchange column chromatography and Sephadex-gel filtration. The specific activity of the enzyme preparation was about 845 units mg of protein. The recovery of activity was 29%. Only one activity band was shown by the polyacrylamide gel electrophoresis.