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应用科学学报 ›› 1984, Vol. 2 ›› Issue (2): 178-183.

• 论文 • 上一篇    下一篇

高活性牛肠碱性磷酸酶的制备

蒋益众1, 邱耀进1, 杨曜中2, 郭春祥3   

  1. 1. 上海科技大学;
    2. 华东化工学院;
    3. 上海医学化验所
  • 收稿日期:1982-04-27 出版日期:1984-06-30 发布日期:1984-06-30

THE PREPARATION OF HIGH ACTIVITY ALKALIN PHOSPHATASE FROM CALF INTESTINAL MUCOSE

JIANG YIZHONG1, QIOU YAOJIU1, YANG YANZHONG2, QUO CHUNXIANG3   

  1. 1. Shanghai University of Science and Technology;
    2. East China Institute of Chemical Technology;
    3. Shanghai Medical Laboratory
  • Received:1982-04-27 Online:1984-06-30 Published:1984-06-30

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86

被引次数

1

摘要： 本文叙述一种从小牛肠粘膜制备碱性磷酸酶的方法.主要包括用正丁醇提取,丙酮和硫酸铵分级,DEAE纤维素离子交换层析和Sephadex凝胶过滤.所得制剂比活为845单位/毫克蛋白;活力回收29%;聚丙烯酰胺凝胶电泳显示单一性活力区带.

Abstract: This paper presents a method for the preparation of alkaline phosphatase (EC 3.1.3.1) from calf intestinal mueose. The method involves extraction of the mucose with butanol, fractionation with acetone and then (NH4)2SO4, DEAE-cellulose ion-exchange column chromatography and Sephadex-gel filtration. The specific activity of the enzyme preparation was about 845 units mg of protein. The recovery of activity was 29%. Only one activity band was shown by the polyacrylamide gel electrophoresis.