酶生物传感器测定鱼类鲜度的研究

应用科学学报 ›› 1996, Vol. 14 ›› Issue (3): 358-363.

酶生物传感器测定鱼类鲜度的研究

彭图治¹, 杨丽菊¹, 李惠萍¹, 沈莲清²

1. 杭州大学;
2. 杭州商学院

收稿日期:1994-09-15 修回日期:1994-11-25 出版日期:1996-09-30 发布日期:1996-09-30
作者简介:彭图治:教授,杭州大学化学系,杭州 310028
基金资助:
浙江省自然科学基金资助项目

STUDIES ON THE ENZYME BIOSENSOR FOR EVALUATING FISH FRESHNESS

PENG TUZHI¹, YANG LIJU¹, LI HUIPING¹, SHEN LIANQING²

1. Hangzho University;
2. Hangzhou Institute of Commerce

Received:1994-09-15 Revised:1994-11-25 Online:1996-09-30 Published:1996-09-30

摘要/Abstract

摘要： 该文以戊二醛为交联剂,将黄嘌呤氧化酶直接固定在丝蛋白做孔膜上,以平面圆盘铂电极为基础电极,研制成黄嘌呤氧化酶电极,检测酶催化反应中过氧化氢的产生量.该电极用酶量极少,对次黄嘌呤的检测下限达1×10^-7mol/L.用此电极对鱼新鲜度进行了测定,与食品分析中测定鱼类新鲜度的常规方法——挥发性盐基氮法和细菌菌落总数法比较,发现存在着很好的相关性,并且初步提出了以次黄嘌呤和黄嘌呤总量为指标判断鱼类新鲜度的范围.

关键词: 次黄嘌呤, 黄嘌呤, 细菌菌落总数, 挥发性盐基氮, 生物传感器, 鱼类新鲜度

Abstract: A sensitive hypoxanthine biosensor was constructed using xanthine oxidase and a plate platinum electrode. The enzyme was conveniently immobilized on a silk protein membrane by glutaraldehyde. The electrode detected the hydrogen peroxide released during the enzymatic reaction. The electrode used very small quantity of enzyme and its detection limit of hypoxanthine was 1×10^-7 mol/L When applied to the determination of the freshness of fish, the results obtained agreed well with those obtained by the traditional methods——the method of total volatile basic nitrogen and the method of total colonies of microbe.

Key words: biosensor, hypoxanthine, total colonies of microbe, freshness of fish, xanthine, total volatile baaic nitrogen

彭图治, 杨丽菊, 李惠萍, 沈莲清. 酶生物传感器测定鱼类鲜度的研究[J]. 应用科学学报, 1996, 14(3): 358-363.

PENG TUZHI, YANG LIJU, LI HUIPING, SHEN LIANQING. STUDIES ON THE ENZYME BIOSENSOR FOR EVALUATING FISH FRESHNESS[J]. Journal of Applied Sciences, 1996, 14(3): 358-363.