English

应用科学学报 ›› 1999, Vol. 17 ›› Issue (1): 14-17.

• 论文 • 上一篇    下一篇

丝蛋白膜免疫传感器的研制及临床分析应用

彭图治1, 祝方猛1, 杨丽菊1, 程琼1, 唐婷1, 陈建勇2   

  1. 1. 杭州大学;
    2. 浙江丝绸工学院
  • 收稿日期:1997-09-07 修回日期:1997-12-29 出版日期:1999-03-31 发布日期:1999-03-31
  • 基金资助:
    中科院电分析化学开放研究实验室和浙江省自然科学基金资助项目

Investigations of Silk Fibroin Electrochemical Immunosensor and Its Application in Clinical Analysis

PENG TUZHI1, ZHU FANGMENG1, YANG LIJÜ1, CHENG QIONG1, TANG TING1, CHEN JIANYONG2   

  1. 1. Department of Chemistry, Hangzhou University, Hangzhou 310028;
    2. Zhejiang Institute of Silk, Hangzhou 310033
  • Received:1997-09-07 Revised:1997-12-29 Online:1999-03-31 Published:1999-03-31

PDF

89

被引次数

19

摘要： 报道了一种可用于临床测定甲胎蛋白的电化学免疫传感器.该传感器采用蚕丝蛋白膜作为固定甲胎蛋白抗体的载体,Ag-AgCl电极作为换能器,直接电位法测定.研究了蚕丝蛋白膜的制备,蚕丝蛋白膜固定甲胎蛋白抗体的三种方法:盐酸活化法、叠氮法、重氮法,以及这些固定方法用于免疫传感器后的结果及比较.其中以盐酸活化法的效果最好,该传感器测定甲胎蛋白的最低检测浓度可达到5μg/L,标准曲线范围在10~300μg/L,相关系数为0.9997.丝蛋白膜免疫传感器具有灵敏度高、制备和操作简便、样品用量少、电极稳定等优点.在临床分析中,样品的结果与放射免疫法相符.

关键词: 蚕丝蛋白膜, 免疫传感器, 固定化方法, 甲胎蛋白

Abstract: In this paper, the preparation of silk fibroin and the ways of immobilizing antibody on silk fibroin are investigated for electrochemical immunosensor. Cross-linking after hydrochloric acid activation, diazo, azide and cross-linking after hydroxylamine activation method are tested for immobilization. In comparison of diazo and azide me thods, the best reponse is obtained when the silk fibroin is activated by hydrochloric acid. The silk fibroin immunosensor is sensitive when measuring α -fetoprotein and the detection limit of 5 μg/L is found. It showes linear response over the concentration range of 10~300 μg/L with correlation coefficient 0 9996. When it is applied to clinical sample analysis, the results are satisfactory.

Key words: α-fetoprotein, immunosensor, immobilization, silk fibroin