用比色法测定固定化葡萄糖氧化酶活性的经验公式

应用科学学报 ›› 1984, Vol. 2 ›› Issue (3): 227-232.

用比色法测定固定化葡萄糖氧化酶活性的经验公式

华家栋, 张宪康, 杨德荣

上海科学技术大学

收稿日期:1982-03-05 出版日期:1984-09-30 发布日期:1984-09-30

A PRELIMINARY REPORT FOR DETERMINING ACTIVITY OF IMMOBILIZED GLUCOSE OXIDASE BY COLORIMETRY

HUA JIADONG, ZHANG XIANKANG, YANG DERONG

Shanghai University of Science and Technology

Received:1982-03-05 Online:1984-09-30 Published:1984-09-30

摘要/Abstract

摘要： 用包埋法得到的固定化葡萄糖氧化酶,我们发现很难找到一个合适的体系,可用比色法从初速度测定它的活性.提出了一个模拟体系,并得到了该体系的吸收光密度与活性的校正曲线,以解析式A=A₀(1-e^-β[E])表示之.式中A为吸收光密度,[E]为葡萄糖氧化酶浓度(活性单位/毫升),A₀和β在给定体系下为常数.测得固定化葡萄糖氧化酶在该体系下的A值,便可从该式计算它的活性.

Abstract: It was difficalt to find a suitable system to determine the activity of immobilized glucose oxidase which was produced from entrapping enzyme by means of colorimetry from initail velocity assay. A two-step "Simulate system" and a convenient calibration formula A=A₀(1-e^-β[E]) were given in this paper. Where a was absorbed optical density,[E] was the concentration of the immobilized glucose oxidase, A₀ and β were constants under a given experimental condition. The more correct activity of immobilized glucose oxidase might be obtained by using the formula mentioned above.

华家栋, 张宪康, 杨德荣. 用比色法测定固定化葡萄糖氧化酶活性的经验公式[J]. 应用科学学报, 1984, 2(3): 227-232.

HUA JIADONG, ZHANG XIANKANG, YANG DERONG. A PRELIMINARY REPORT FOR DETERMINING ACTIVITY OF IMMOBILIZED GLUCOSE OXIDASE BY COLORIMETRY[J]. Journal of Applied Sciences, 1984, 2(3): 227-232.