光交联聚乙烯醇水凝胶及青霉素敏生物传感器研究

应用科学学报 ›› 1996, Vol. 14 ›› Issue (1): 91-96.

光交联聚乙烯醇水凝胶及青霉素敏生物传感器研究

贾德民, 庞永新, 郭柏平, 李晖

华南理工大学

收稿日期:1994-03-31 修回日期:1994-12-04 出版日期:1996-03-31 发布日期:1996-03-31
作者简介:贾德民:教授,华南理工大学高分子材料科学与工程系,广州 510041

STUDY ON THE PHOTOCROSSLINKED POLY (VINYL ALCOHOL)HYDROGELS AND PENICILLIN SENSITIVE BIOSENSOR

JIA DEMIN, PANG YONGXIN, GUO BOPING, LI HUI

South China University of Technology

Received:1994-03-31 Revised:1994-12-04 Online:1996-03-31 Published:1996-03-31

摘要/Abstract

摘要： 合成了一种光交联聚乙烯酸(PVA-QQ)水凝胶.着重讨论了该水凝胶固定化β-内酰胺青霉素酶膜及其与离子敏感场效应管(pH-FET)结合构成的青霉素敏生物传感器的性能.结果表明,PVA-QQ水凝胶固定化酶膜具有较高的活性及优良的温度、酸碱度、时间及力学稳定性.由该酶膜制备的青霉素敏FET的响应时间小于60s,灵敏度大于3mV/mM,线性响应范围为0~16mM.

关键词: 生物传感器, 聚乙烯醇, 青霉素, 固定化酶

Abstract: A novel photocrosslinked poly(vinyl alcohol) hydrogel was prepared and applied to entrap β-lactam penicitllinase.The properties of the hydrogel and immobilized enzyme were thoroughly investigated. Results show that the hydrogel is very suitable for the immobllization of β-lactam penieillinase.The stabilities of the immobilized enzyme to PH value of the medium, temperature and storage are better than those of the free β-laotam penioilllnaee. Also the immobilized enzyme membrane is connected to a pH-sensitive field effect transistor to form a penieillin-sensitive biosensor. The specifications of the resulting biosensor are as follows:response time less than 60sec, sensitivity to penicillin larger than 3mV/mM,1inear response range of 0~16mM, and very good storage stability.When it is applied to determine the penicillin content of the fermenting mixture during the production process, the results are found to conform to those obtained by the conventional iodine titration.

Key words: penicillinase, biosensor, poly (vinyl alcohol), immobilized enzyme

贾德民, 庞永新, 郭柏平, 李晖. 光交联聚乙烯醇水凝胶及青霉素敏生物传感器研究[J]. 应用科学学报, 1996, 14(1): 91-96.

JIA DEMIN, PANG YONGXIN, GUO BOPING, LI HUI. STUDY ON THE PHOTOCROSSLINKED POLY (VINYL ALCOHOL)HYDROGELS AND PENICILLIN SENSITIVE BIOSENSOR[J]. Journal of Applied Sciences, 1996, 14(1): 91-96.