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应用科学学报

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交变磁场对含盐溶液冰晶形成的影响

陈照章 王恒海 黄永红 徐晓斌   

  1. 江苏大学 电气信息工程学院，江苏 镇江 212013
  • 收稿日期:2007-05-30 修回日期:2007-12-03 出版日期:2008-03-31 发布日期:2008-03-31

Influence of Alternated Magnetic Field on Ice Crystal Formation Process in Aqueous Solution

CHEN Zhao-zhang, WANG Heng-hai, HUANG Yong-hong, XU Xiao-bin   

  1. School of Electrical and Information Engineering, Jiangsu University, Zhenjiang 212013, China
  • Received:2007-05-30 Revised:2007-12-03 Online:2008-03-31 Published:2008-03-31

被引次数

17

摘要： 生物组织的低温无损保存是低温生物学的一个重要课题。本文采用串联谐振方法产生交变磁场，在低降温速率条件下，研究交变磁场对KMnO4溶液中冰晶形成过程的影响。发现50kHz交变磁场对该溶液中冰晶的生长具有显著的抑制作用，并形成了含盐冰。本研究成果对容易实现的低冷冻速率下生物组织的低温无损保存具有重要意义，适合实际应用。

关键词: 低温保存, 冰晶, 串联谐振, 虚拟仪器

Abstract: Nondestructive cryogenic preservation has been an important research topic in cryobiology. Organisms are mostly composed of aqueous solution. Common methods involve the use of an ultra-rapid cooling rate or a very high concentration of cryoprotectants. Unfortunately, adding cryoprotectants introduces impurities for biomaterials themselves and ultrarapid freezing is very difficult to obtain the same cooling rate for the surface and interior of big samples. The influence of ice crystal formation process in KMnO4 solution by alternated magnetic field was studied under the condition of low cooling rate. Alternated magnetic field was produced based on series resonance. This study shows that 50 kHz alternated magnetic field can suppress ice crystal growth in the solution, and salty ice formed. The result of low freezing rate cryopreservation easy achieved is of great significance, and useful in practical applications.

Key words: cryopreservation, ice crystal, series resonance, virtual instrument